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Objective:To establish a colon cancer cell line which overexpressing LINC01018 stably and study its biological characteristics.Methods:The expression of LINC01018 in HCoEpiC and HT-29 cells were detected by real time fluorescence quantitative polymerase chain reaction (qRT-PCR). HT-29 cells were infected with LINC01018 overexpression lentivirus to screen and establish HT-29 cell lines which overexpressing LINC01018 stably. The effect of LINC01018 on the proliferation, invasion and migration of HT-29 cells were detected by cell counting kit-8 (CCK-8) assay and Transwell assay separately. The expression of CDK6 and matrix metalloproteinase-2 (MMP-2) in HT-29 cells was detected by Western blot.Results:The expression of LINC01018 in HT-29 cells was significantly lower than that in the human colonic epithelial cells (HCoEpiC). HT-29-L18 cell lines which overexpressing LINC01018 stably was screened successfully. Overexpression of LINC01018 significantly inhibited the cell proliferation, invasion and migration, and reduced the protein expression of CDK6 and MMP-2 in HT-29 cells.Conclusions:The expression of LINC01018 was decreased abnormally in colon cancer cells. Up-regulation of LINC01018 expression can inhibit the proliferation, invasion and migration of colon cancer cells, which may be related to CDK6 and MMP-2.
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Objective:In order to establish a painless delivery detection system in plateau area, promote the concept of painless delivery, reduce the cesarean section rate, the clinical study of painless childbirth in Tibet was carried out.Methods:150 primiparas in Lhasa People's Hospital from January 2018 to Juen 2019 were prospectively collected, which were randomly divided into sufentanil and ropivacaine groups (group A, n=50) and fentanyl and ropivacaine (group B, n=50) and the control group without analgesia (group C, n=50); The general conditions of the patients before and after delivery were collected. The visual analogue scale (VAS) score was used to observe and record the delivery process and results. The Apgar score of newborns was performed, and the umbilical artery blood was taken for blood gas analysis. Results:The first and second stage of labor in group A and B were shorter than those in group C ( P<0.05); there was no difference in the third stage of labor and postpartum bleeding between the three groups ( P>0.05). The VAS scores of groups A and B were lower than that of group C ( P<0.05), while there was no difference between group A and group B ( P>0.05). There was no difference in pH value of umbilical artery blood between the three groups ( P>0.05). The umbilical artery blood PO 2 of group A and group B was higher than those of group C ( P<0.05), while there was no difference between group A and group B ( P>0.05). The umbilical artery blood PCO 2 of group A and group B was lower than that of group C( P<0.05), while there was no difference between group A and group B ( P>0.05). The cesarean section rate in group C was higher than that in groups A and B ( P<0.05). There was no difference in fetal heart rate, fetal distress, and neonatal asphyxia among the three groups ( P>0.05). There was significant difference in the overall incidence of adverse conditions in the three groups ( P<0.05). The Apgar scores of the newborns in groups A and B were higher than those in the C group at 1 min postpartum ( P<0.05), while there were no difference in the Apgar scores among the three groups at 5 and 10 min postpartum ( P>0.05). Conclusions:Painless delivery in plateau area can reduce the pain of delivery and the rate of cesarean section, and has high safety and reduce the adverse effects on mothers and infants, which is worthy of clinical promotion.
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Objective To renovate angiography in identifying portal vein anatomy during transjugular intrahepatic portosystemic shunt (TIPS) procedures,saving the time of TIPS procedures,decreasing the risk of the complications of the post-procedure.MethodsThe difference between the Wedge hepatic venography with Carbon Dioxide in 6 cases and Inferior Mesenteric artery angiography in 7 cases during TIPS procedures were compared in the identification of portal vein anatomy.The quality of images,their effects on the procedures,the complications and the recovery post-procedure were evaluated.Results Using CO2,the portal veins were opacified in all 6 cases.TIPS procedures succeeded in all cases except 1 case because of poor coagulation function.Using Inferior Mesenteric artery angiography,the portal veins were opacified in al1 7 cases.TIPS procedure succeeded in all cases except 1 case because of chronic portal occlusion.Puncture-site hematoma occurred in 1 case after TIPS procedure.ConclusionWedge hepatic venography with Carbon Dioxide is superior,safer and more convenient than Inferior Mesenteric Artery angiography in identifying portal vein anatomy during TIPS.
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Objective To study characteristics of hospital infection and bacterial drug-resistance in our new Intensive Care Unit (ICU) to provide evidence to clinical rational use of drugs. Methods The data of 142 patients in our ICU last year were analyzed retrospectively, and compared to other inpatients treated in general ward during the same time period. Results Among the 142 ICU inpatients,31 times of hospital infection occurred in 22 patients,gave rise to a infection rate of 15.49% and case time infection rate of 21.83% in ICU; but among the 15 978patients treated simultaneously in general ward,413 times of hospital infection occurred in 324 inpatients, gave rise to a infection rate of 2. 03% and case time infection rate of 2. 58% outside of ICU. The hospital infection rate and case time infection rate were significantly different between the ICU and general ward (P ＜ 0. 01 ). Among the ICU infections, the lower respiratory tract infection accounted for 70. 96% ;the main pathogens were Gram-negative bacilli (65. 12% ) and Gram-positive bacilli (23.26%), follwed by fungus (11.63%). The most common bacteria were Escherichia ( 32. 65% ) and Staphylococcus ( 11.63% ). Gram-negative microorganisms were highly sensitive to cefoperazone/sulbactam,imipenem, meropenem and piperacillin/tazobactam; all Gram-positive microorganisms were sensitive to vancomycin,linezolid and teicoplanin. Conclusions The constitution,distribution and drug-resistance of pathogens in new ICU are similar to previous domestic reports. Gram-negative organisms are the main photogenic bacteria of hospital infection in ICU, and Escherichia is the most popular type in nosocomial infection.
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Objective To evaluate the relationship between amniotic fluid (AF) epidermalgrowth factor (EGF) concentration and fetal pulmonary maturation. Methods Lecithin － sphin-gomyelin ratio (L/S) and EGF were measured in amniotic fluid from women(n = 138,21～42 weeks'gestation) undergoing amniocentesis by TLC and RIA. ResultsThe AF L/S ratio and EGF concen-tration increased linearly with Gestational age(GA) ( r = 0.8t3 and 0.87, respectively, P < 0.01 ).There was a strong correlation between the AF L/S ratio and EGF( r = 0.76, P < 0. 001 ). Conclu-sionThere are a confirmed relationship between AF EGF levels and fetal pulmonary maturation.
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Objective To study the relationship between the type of histopathology and prognosis of contained mucous adenocarcinoma(CMAC) of stomach and its clinical significance. Methods Eighty-seven cases of CMAC of stomach were observed by means of pathomorphology, histochemistry, immunohistochemistry and follow up etc. Results The malignant behavior of the cancer was significant difference according to the nature and quantity of the CAMC and the primary pathological type of the cancer.The clinical type in simple mucous carcinoma was mainly Borrmann type I;in tubular papilla mucous adenocarcinoma was mainly Borrmman type II; in signet-ring cell mucous carcinoma was mainly Borrmman type IV,and in mixed cell mucous carcinoma was mainly Borrmman type III. There was significant difference in the lymphatic metastasis and survival rate between the 4 groups. Conclusions Correct differentiation of the pathological type of contained mucous adenocarcinoma of stomach is important for guading the treatment and predicting the prognosis.Cathepsin D expression can help for understanding the biological behavior of CMAC of the stomach.